ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
TWO BIOACTIVE COMPOUNDS AND A NOVEL CHALCONE SYNTHASELIKE ENZYME IDENTIFIED IN GERBERA HYBRIDA |
| Authors: | S. Koskela, P. Elomaa, Y. Helariutta, P. Söderholm, P. Vuorela |
| Keywords: | Botrytis cinerea, CHS, gerbera, gerberin, parasorboside |
| Abstract:
We have identified a new chalcone synthase (CHS) -like enzyme, 2-pyrone synthase (2PS), in Gerbera hybrida var. Regina (Asteraceae). While 2PS has amino acid sequence identity of ~70% to CHS enzymes, it has a novel substrate specificity and uses acetyl-CoA as a starter substrate. Transgenic 2PS antisense gerbera plants with very low 2PS activity lack two abundant secondary metabolites found in control plants, gerberin and parasorboside. The antisense plants show susceptibility for the mold Botrytis cinerea and insect pests. Statistical analyses revealed significant difference between resistent and sensitive G. hybrida varieties when concerning the total amount of gerberin and | |
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