ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
EFFECT OF PPM ON ALKALOID PRODUCTION IN NARCISSUS CONFUSUS SHOOT-CLUMPS |
| Authors: | R. Colque, F. Viladomat, J. Bastida, C. Codina |
| Keywords: | Amaryllidaceae, antibiotic, biomass, galanthamine, liquid culture, secondary metabolism |
| Abstract:
The Plant Preservative Mixture (PPM) has been reported to have no effect on plant cell development. A study was run to observe the effects of PPM on the physiological development and production of galanthamine (an alkaloid used in the treatment of Alzheimer’s disease) and other alkaloids in Narcissus confusus shoot-clumps. Liquid-shaked medium with concentrations of PPM from 0 (control) to 10 ml l-l were used and the explants were cultured for 4 weeks, being subcultured in the 2nd week. The alkaloid release to the medium and the biomass gain were measured in both steps, and the alkaloid concentration in tissue was measured at the end of the experiment. No effects on physiological development and the biomass increase were observed in all the PPM concentrations. Although the PPM effect on most of the alkaloid content in tissue was irregular, the highest level of galanthamine was obtained with 0.5 ml l-l of PPM. A gradual decrease in the galanthamine levels was also observed with higher PPM doses. Quantification of the galanthamine released to the medium showed that in the control, the total released was almost the same as the level obtained with 0.5 ml l-l of PPM. In all cases the control did not show significant differences with respect to the recommended dose levels in galanthamine release and production. | |
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