ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
IDENTIFICATION OF SECONDARY METABOLITES IN IN VITRO CULTURE OF AMMI MAJUS TREATED WITH ELICITORS |
| Authors: | A. Królicka, E. Lojkowska, I. Staniszewska, E. Malinski, J. Szafranek |
| Keywords: | bishops weed, callus culture, cell culture, furanocoumarins, hairy roots |
| Abstract:
The work was concentrated on the production of secondary metabolites in callus, cell suspension and hairy roots of Ammi majus by exposing them to abiotic elicitors: suspension of silicon dioxide (SiO2), jasmonic acid and biotic elicitors: autoclaved lysats of bacteria – Enterobacter sakazaki, Erwinia chrysanthemi and fungal cell wall compound – scleroglucan. Thin-Layer Chromatography and Gas Chromatography of the chloroform and methanol extracts of elicited callus, cell suspension and hairy root cultures indicated accumulation of umbelliferone, a biogenetic precursor of furano-coumarins in elicited tissues. The highest induction of umbelliferone was observed after treating the callus cultures with SiO2, but the highest productiveness of umbelliferone was obtained in case of hairy root cultures with the same abiotic elicitor. Treatment of the cell suspension with scleroglucan and E. chrysanthemi induced production of linear furanocoumarin – marmesinin. Using Gas Chromatography - Mass Spectrometry scopoletin was identified in callus cultures of A. majus elicited with E. sakazaki. | |
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