ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
GENETIC TRANSFORMATION OF SWEET POTATO (Ipomoea batatas (L.) LAM.) BY AGROBACTERIUM TUMEFACIENS |
| Authors: | M. Otani, Y. Wakita, T. Shimada |
| Keywords: | transformation, sweet potato, Agrobacterium tumefaciens, transgenic plants |
| Abstract:
Transgenic sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA101/pIG121-Hm used in the present study contained a binary vector with genes for gusA and hygromycin resistance (hpt). Around ten hygromycin resistant cell clusters were produced from 1 g fresh weight of the infected embryogenic calli. The hygromycin resistant plantlets were regenerated from 53.1% of the hygromycin-resistant calli. Histochemical GUS assay and Southern hybridization analysis indicated that these plants were stably transformed with a copy number of introduced genes of one to three. Transgenic plants grew normally and formed storage roots after 3 months of cultivation in a green house. We have introduced some agronomically important genes by using our transformation system. | |
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