ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
AGROBACTERIUM-MEDIATED TRANSIENT EXPRESSION OF β-GLUCURONIDASE AND LUCIFERASE GENES IN LIMONIUM SINUATUM |
| Authors: | M. Kimizou, Y. Yamamoto, T. Iwasaki, S. Ohki |
| Keywords: | statice, Thidiazuron, leaf explant, hygromycin resistance, intron |
| Abstract:
We have developed an Agrobacterium-mediated transformation system for Limonium sinuatum. The shoot regeneration was observed on thidiazuron (TDZ)-containing medium. Shoot-forming capacity of this species depended on the cultivar. About 40 % of the explants of' cv. PF142 regenerated shoots on 0.1 µM TDZ medium. Luciferase (LUC) and  -glucuronidase (GUS) genes were introduced and transient expression of these gene were confirmed.
Stable expression of the GUS gene was also confirmed, but shoot formation was strongly inhibited by hygromycin B, of which resistant gene was used as a selection marker.
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