ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
FIRST RESULTS OF PELARGONIUM SHOOT APEX CRYOPRESERVATION |
| Authors: | A. Grapin, C. Hupel, C. Marche, N. Dorion |
| Keywords: | encapsulation-dehydration, alginate beads, silica-gel dehydration, germplasm conservation, Pelargonium peltatum |
| Abstract:
In order to guarantee a safe long-term conservation of a Pelargonium collection a cryopreservation process using encapsulation-dehydration was tested on shoot apices. Explants of Pelargonium peltatum ‘Ville de Paris lilas’ were excised from plants grown in a greenhouse. Alginate beads, each containing one apex, were precultured for 48h in a liquid medium progressively enriched in sucrose, then dehydrated and directly freezed in liquid nitrogen. The best results (70-75% survival) were obtained using: 1M sucrose and dehydration time of 5h, and 1.25M sucrose and dehydration time of 4h. A 64h period of culture before apex encapsulation decreased shoot-tip survival. A higher survival rate was obtained by extracting the shoot apices from the beads 3 days after rewarming. | |
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