ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
GENETIC VARIABILITY OF ANTHER DONOR VERSUS SPONTANEOUS DOUBLED HAPLOID DESCENDENTS AND COLCHICINE INDUCED DOUBLED HAPLOID SWEET PEPPER (Capsicum annuum L.) LINES |
| Authors: | A. Gémesné Juhász, M. Petus, G. Venczel, L. Zatykó, G. Gyulai, M. Cséplö |
| Keywords: | Capsicum annuum, spontaneous doubled haploid, colchicine treated doubled haploid, RAPD, SSR-ISSR PCR analysis |
| Abstract:
Haploid (n) and doubled haploid (DH) plants were developed in anther culture of sweet pepper (Capsicum annuum L.). Regenerants were analyzed by flow cytometry for haploid (n = 12) and spontaneous doubled haploid (2n = 24) genomes. Haploid plants were forwarded to colchicine-treatment for induced doubled haploid (2nˇ) plant production. Molecular polymorphism of anther donor plants (2n), the haploid regenerants (n), the spontaneous (2n) and induced (2nˇ)-DH plants were analysed by RAPD-, SSR- and ISSR-PCR. The analysis of anther-donor plants compared to DH-descendents showed an unexpectedly wide range of molecular polymorphism. Our results suggest that genetic changes occurring during meiotic recombination is higher than those of occurring during colchicine-induced genomic duplication. | |
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