ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
VITRIFICATION OF SHOOT LIPS, NODAL SEGMENTS AND EMBRYOGENIC TISSUE OF OLIVE (olea europaea L.) FOR GERMPLASM CRYOPRESERVATION |
| Authors: | C. Benelli, A. De Carlo, M. Lambardi, P.T. Lynch |
| Keywords: | cryopreservation, olea europaea, vitrification, encapsulation-dehydration |
| Abstract:
The study explores the possibility of olive (Olea europaea L.) germplasm cryopreservation, using both the vitrification and the encapsulation-dehydration procedures, followed by one-step freezing in liquid nitrogen. When shoot tips of olive (cv Frantoio) were incubated at 0°C in the PVS2 vitrification solution for 60 min, a 15% survival was recorded after direct immersion in liquid nitrogen and thawing at 40°C. More than 90% of encapsulated shoot tips and nodal segments survived after a 2-day preculture in 0.5 M-sucrose OM medium and a 4-hour exposure to silica gel, but the treatment was not effective in the protection of explants during ultra-freezing. Best results were obtained with olive embryogenic tissue (cv Canino), as 38% of samples survived to cryopreservation and showed enhanced morphogenicity. | |
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