ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
CRYOPRESERVATION OF WOODY SPECIES BY VITRIFICATION OF SHOOT TIPS AND EMBRYOGENIC TISSUE |
| Authors: | M. Lambardi, A. De Carlo, C. Benelli, G. Bartolini |
| Keywords: | cryopreservation, vitrification, germplasm, somatic embryogenesis |
| Abstract:
The technique of vitrification/one-step freezing in liquid nitrogen has been applied to the cryopreservation (-196°C) of shoot tips and embryogenic tissue of various woody species, i.e., poplar (Populus spp.), plum (Prunus domestica), olive (Olea europaea), and horsechestnut (Aesculus hippocastanum). High percentages of shoot-tip survival were obtained with Populus alba (90%), Populus canescens (54%) and plum (47%) when the explants were exposed to the PVS2 vitrification solution (30% glycerol, 15% ethylene glycol, 15% DMSO in 0.4 M-sucrose MS medium) at 0°C for 60 min (poplars) or 90 min (plum), after which they were directly plunged into liquid nitrogen and thawed at 40°C. Lower post-thaw survivals were obtained with shoot tips of Populus nigra (22%) and olive (15%). Several portions of embryogenic tissue of both olive and horsechestnut survived cryopreservation, maintaining or even enhancing their morphogenic potential. These results indicate that the vitrification procedure is a promising technique for the cryopreservation of a wide range of tissues from in vitro cultured woody plants. | |
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