ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
INTRODUCTION OF THE ATMOSPHERIC NITROGEN FIXING ABILITY TO PLANTS: DIRECT BACTERIUM BOMBARDMENT |
| Authors: | E. Preininger, K. Boka, P. Korányi, I. Gyurján, J. Zatykó |
| Abstract:
An artifical symbiosis was established between nitrogen fixing Azomonas insignis and strawberry (Fragaria x ananassa). The partnership was created by in vitro techniques through callus induction and organogenesis. The basis of this partnerships is the bacterial dependence on the plant’s metabolic activity, using maltose in the medium as a carbon and energy source which can be utilized by the plant cells only. For successful and high frequency introduction of bacteria to the plant tissues, biolistic gun method was used. On the basis of the DNA transfer method, Azotobacter vinelandii bacteria were delivered directly into strawberry tissues by the particle bombardment. This was the first time to use living bacteria as microprojectiles for bombardment of plant tissues. The treatment was successful, the presence of bacteria in the developing callus tissue and regenerated plants were detected by light and electron microscopy. | |
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