ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
A COMPARISON OF DIRECT AND INDIRECT SOMATIC MORPHOGENESIS FOR THE PRODUCTION OF TRANSGENIC SUGARCANE (SACCHARUM SPP. HYBRIDS) |
| Authors: | S.J. Snyman, B.I. Huckett, M.P. Watt, F.C. Botha |
| Keywords: | 2,4-D concentration, production costs, target material, somatic embryogenesis |
| Abstract:
The conventional genetic transformation protocol for sugarcane employs microprojectile bombardment of embryogenic callus. We report an approach involving bombardment of leaf explants, followed by direct somatic embryogenesis. The technique involves gene delivery to immature leaf roll segments cultured for only 2 weeks on MS with 0.3 mg 1-1 2,4-D. When this method was compared to the conventional one by producing transgenic plants containing the pat gene, PCR-determined transformation efficiencies were 72 and 100%, respectively. However, the newly-developed protocol reduced plantlet production time and number of subcultures, thereby reducing the cost of transgenic plant production from US$ 33 to US$ 2.7 per plant. | |
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