ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
INCREASED RESISTANCE TO ERWINIA AMYLOVORA EXHIBITED BY TRANSGENIC 'ROYAL GALA' APPLE (MALUS x DOMESTICA BORKH.) SHOOTS, CARRYING A MODIFIED CECROPIN MB39 GENE |
| Authors: | Q. Liu, J. Ingersoll, L. Owens, S. Salih, R. Meng, F. Hammerschlag |
| Keywords: | Agrobacterium tumefaciens, fire blight, gene transfer |
| Abstract:
Transgenic 'Royal Gala' apple (Malus x domestica Borkh.) shoots were obtained by Agrobacterium-mediated gene transfer using a vector with T-DNA encoding a modified cecropin gene MB39 joined to a secretory coding sequence from barley α-amylase, and placed under the control of a wound-inducible tobacco osmotin promoter. The integration of the cecropin MB39 gene into apple was confirmed by Southern blot analysis. Three of the seven transgenics exhibited 2.5- to 3.3-fold more resistant to Erwinia amylovora than the non-transformed 'Royal Gala' control, demonstrating the potential of introducing the cecropin gene to increased levels of disease resistance. | |
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