ISHS Acta Horticulturae 560: IV International Symposium on In Vitro Culture and Horticultural Breeding
CHROMOSOME NUMBER DEPENDENT GENOME SIZE AND RAPD FINGERPRINTING DIAGNOSTICS FOR GENETIC INTEGRITY OF ENHANCED AXILLARY BRANCHING-DERIVED PLANTS OF TEN FOREST TREE SPECIES |
| Authors: | V. Rani, A. Parida, S.N. Raina |
| Keywords: | Micropropagation, Somaclonal variation, Genotype specificity, Populus, Eucalyptus, Anogeissus, Alnus, Prosopis, Wrightia, Tecomelaa |
| DOI: | 10.17660/ActaHortic.2001.560.108 |
| Abstract:
Rapid comparisons of the chromosome number by genome size determination were made in the explant source and 1400 enhanced axillary branching-derived plants of Populus deltoides clones, P. ciliata, Eucalyptus tereticornis, E. camaldulensis, Anogeissus pendula, Alnus nepalensis, Prosopis cinerarea, Wrightia tomentosa, W. tinctoria and Tecomella undulata. The estimated DNA amounts in the meristematic zones of root and shoot tips of each of the plants, collected at various cycles of tissue culture from multiplication to field transfer, complemented with the DNA amount of the zygotic chromosome number (2n) of the respective species. RAPD fingerprinting, however, revealed polymorphism among micropropagated plants of two P. deltoides clones. This emphasizes the need for genetic analysis of micropropagated plants by more than one approach before a specific protocol is released for mass multiplication. | |
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