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ISHS Acta Horticulturae 56: Symposium on Juvenility in Woody Perennials

FLORAL TRANSFORMATION OF VEGETATIVE PLANTS BY SOLUTIONS CONTAINING DEOXYRIBONUCLEIC ACID EXTRACTED FROM STEMS IN A FLORAL STATE

Author:   W. L. Wardell
Abstract:
Experiments have been performed to investigate primarily the capabilities of solutions containing deoxyribonucleic acid (DNA) extracted from tobacco (Nicotiana tabacum L. cv. Wisconsin 38) plants to effect flowering. Data are reviewed which indicate that solutions containing DNA extracted from stems in a floral state effect floral transformation of vegetative plants and under certain circumstances effect phenotypic modifications in flowers of treated plants as well as their offspring, whereas solutions containing DNA extracted from stems or leaves in a vegetative state possess no floral activity. Treating with deoxyribonuclease I (DNase I) a solution of DNA extracted from stems in a floral state completely eliminates the floral activity. The possible implications of these findings to the transition of a woody perennial from a juvenile state to a mature state are discussed.

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