ISHS Acta Horticulturae 558: I International Symposium on Litchi and Longan
SHOOT TIP CULTURE AND PRODUCTION OF VIRUS-FREE LONGAN PLANTLETS |
| Author: | J.F. Wang |
| Keywords: | Dimocarpus longan Lour., ‘Longan witches' broom’, virus elimination |
| Abstract:
Four longan cultivars, Dongbi, Bianxiajian, Wulongling and Honghezi, were used as experimental materials. Tips of mature shoots were taken from healthy trees and from those infected with ‘longan witches' broom’. After surface sterilization, the shoot tips were cut into 5-mm pieces and inoculated onto MS, N6, 1/2MS or White media supplemented with various combinations of BA, GA3 and IAA in under conditions, and cultured at 25±1°C with daily illumination of 2000 lux for 8 to 10 h. Germination occurred between 15 and 20 days after inoculation. For subsequent subculture, buds were cut into 5-mm long pieces and cultured on the MS medium with 0.5-1.0 mg/L BA and 0.1-0.5 mg/L IAA. Prolific shoot formation was observed. Meristem tissue, 0.3 to 0.5 mm in length, was cut from the buds induced from diseased explants and subcultured for 6 weeks at alternating temperatures (16 h of 40°C and 8 h of 22°C). Shoots thus regenerated were transferred onto a rooting medium with 0.5 mg/L IBA and 0.1-0.5 mg/L BA. The young rooted plantlets were transplanted from the culture tube into clay pots containing sterilized sand and peat soil (1:1,v/v) and covered with polyethylene bags. Some of the plantlets derived from diseased explants grew normally, and the virus-elimination rate was 30%. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
