ISHS Acta Horticulturae 556: V International Congress on Hazelnut
MICROPROPAGATION OF HYBRID HAZELNUT: MEDIUM COMPOSITION, PHYSICAL STATE AND IRON SOURCE AFFECT SHOOT MORPHOGENESIS, MULTIPLICATION AND EXPLANT VITALITY |
| Authors: | M.N. Nas, P.E. Read |
| Keywords: | Corylus, filbert, tissue culture, sequestrene iron |
| Abstract:
Explants of mature hazelnut plants cultured on liquid Driver and Kuniyuki (DKW) medium produced longer shoots and bigger leaves compared to those cultured on medium gelled with agar. Iron source affected shoot length and leaf color, but the number of shoots produced was not significantly different. When ferrous sulfate (33.8 mg L-1) was used as the iron source, shoot length increased but the leaves were mostly chlorotic. Shoots formed on the medium containing Sequestrene 138 Fe (200 mg L-1) were healthy-looking and dark green while those on the medium with Sequestrene 330 Fe (120 mg L-1) exhibited hyperhydricity. Different medium compositions supplemented with B5 vitamins affected shoot length, shoot number, amount of callus and leaf color. Shoots on Woody Plant Medium (WPM) were longer than those on Nitsch and Nitsch (NN), DKW and Murashige and Skoog (MS) media. The number of shoots per explant was higher on WPM and NN than on DKW and MS. The greenest leaves were on NN and the most chlorotic leaves were on MS media. When chlorotic explants were grown on MS medium in subsequent subcultures, the number of explants surviving decreased dramatically. Explants cultured on WPM and NN media produced more callus than those cultured on DKW and MS media. These results have potential practical applications in micropropagation of hybrid hazelnuts. | |
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