ISHS Acta Horticulturae 556: V International Congress on Hazelnut
NUCLEIC ACID EXTRACTION FROM CORYLUS AVELLANA TISSUES |
| Authors: | N.V. Bassil, J. Davis, A.N. Azarenko, S.A. Mehlenbacher |
| Keywords: | DNA, RNA, PCR, RAPD |
| Abstract:
A protocol for large-scale DNA extraction from young hazelnut leaves and catkins was developed. It is suitable for daily extractions of 200 to 300 samples and requires 2-5 days to complete. About 97% of the DNA samples obtained are amenable to amplification by the polymerase chain reaction. Six methods were compared for RNA isolation. Good quality RNA was successfully isolated from young hazelnut leaves and styles using the RNeasy Plant Kit (Qiagen) with modifications to the manufacturer's protocol. Yet a CTAB (hexadecyltrimethylammonium bromide) method originally used to isolate RNA from pine needles was superior to all five RNeasy Plant Kit-based protocols. | |
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