ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 555: II International Symposium on Edible Alliaceae

EXPRESSED SEQUENCE TAGS FROM BULB ONION

Authors:   J. McCallum, M.J. Havey, J. Lancaster, J. Farrant, S. Clark
Keywords:   RFLP, mapping, cDNA sequencing
DOI:   10.17660/ActaHortic.2001.555.8
Abstract:
To date there is relatively little DNA sequence information from Allium. We have sequenced cDNAs from A. cepa libraries prepared from bulbs, roots and seedlings to identify loci on the A. cepa linkage map and facilitate development of PCR-based expressed sequence tag ( EST) markers. BLAST comparisons with GENBANK sequences indicated 80% of these showed homology with genes of known function or ESTs. Eighteen of sequenced clones were mapped as restriction fragment length polymorphisms (RFLP) and were of abundant transcripts encoded by gene families (CAB proteins, RuBISCO, ubiquitin, elongation factors). EST markers are being developed from selected sequences.
  • Article - full text (enhanced PDF format, 31605 bytes)
  • How to cite this article
  • Translate

Download Adobe Acrobat Reader (free software to read PDF files)

555_7     555     555_9

URL www.actahort.org      Hosted by KU Leuven      © ISHS