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| Authors: | G. Yan, B. Croxford, R. Sedgley |
| Keywords: | Plant breeding, pollen storage, pollen pistil interaction, seed germination, chromosome number, Proteaceae |
Abstract:
More than 500 different interspecific cross combinations from 140 genotypes of 27 Leucadendron species were hand pollinated in 1998-2000 flowering seasons aiming at producing new cultivars suitable for Australian growing conditions.
More than 2,000 flower heads were collected and about 18,000 seeds were extracted.
Over 3,000 seedlings representing most cross combinations have been planted.
A breeding protocol including, selection of parents, pollen collection, assessment and storage, isolation of female flowers, pollination and seed collection and germination has been developed.
Studies of pollen pistil interactions revealed that hybridisation barriers exist among some interspecific cross combinations.
Cytogenetic work indicated that 25 genotypes involving 15 species studied so far have the same chromosome number of 26 with little chromosome size variation.
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