ISHS Acta Horticulturae 551: IX International Symposium on Small Fruit Virus Diseases
APPENDIX I. RECOMMENDED PROCEDURES FOR DETECTION OF VIRUSES OF SMALL FRUIT CROPS |
| Author: | R.R. Martin |
| DOI: | 10.17660/ActaHortic.2001.551.18 |
| Abstract:
The following tables outline the procedures recommended for the detection of viruses of small fruit crops in the genera, Fragaria, Humulus, Ribes, Rubus and Vaccinium. In cases where a test needs validation it is recommended that an additional test be performed on the test plants. These tests that need validation have only been used on one or a few isolates of a virus. Before they can be recommended tests they need to be evaluated on a broad range of isolates from multiple locations. In the case of polymerase chain reaction assays it may be necessary to state primer sequences that have been shown to be useful for detecting a wide range of virus isolates when recommending a test. Only a subset of a virus sequence is likely to be highly conserved and suitable for detecting all strains of a virus. This is also true for monoclonal antibodies that will be recommended for ELISA tests. Some monoclonal antibodies will be strain specific as has been shown for tomato ringspot, tobacco streak, cucumber mosaic etc. In some cases polyclonal antisera must be designated as well since strain specificity can be a problem with some polyclonal antibodies, this is notably so with many nepoviruses. At the meetings in Canterbury at the University of Kent in 2000, plant virologists that work with virus diseases of small fruit crops agreed to work together to validate new tests in order that these new tests can then become recommended procedures for virus testing and used in certification and quarantine programs.
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