|Author: ||M. Cieslinska|
|Keywords: ||Strawberry, Raspberry, Blackberry, Phytoplasma, Strawberry Green petal, Rubus stunt, PCR, RFLP|
A survey of strawberry plantations in some regions of Poland was conducted in 1998 and 1999. Strawberry green petal symptoms were observed on ‘Mara des Bois’, ‘Selva’, ‘Evita’ and ‘Tango’ plants.
Observations conducted in 1998 and 1999 on experimental plantations of Rubus sp. showed the presence of symptoms typical for rubus stunt phytoplasma on the raspberry ‘Royality’, raspberry x blackberry ‘Loganberry’, blackberry ‘Darrow’, as well as on some wild blackberry and raspberry plants. Catharanthus roseus plants were used for transmission of phytoplasma from suspected strawberries.
The polymerase chain reaction (PCR) was applied for the detection of the phytoplasmas in the suspected plants.
DNA fragments of the expected size were amplified from the strawberries ‘Mara des Bois’, ‘Tango’, ‘Selva’, ‘Evita’, infected C. roseus plants and positive controls in the experiments conducted in 1998 with primer pair fU5/rU3. In the 1999 season it was difficult to detect phytoplasma in the suspected strawberries.
The specific DNA fragments were amplified by PCR using fU5/rU3, R16F2/R2 or P1/P7 primer pairs from the extracts of Rubus sp. plants expressing symptoms.
The restriction length polymorphism analyses of the PCR products using AluI and RsaI enzymes allowed to classify the phytoplasma infecting one of the wild blackberry to elm yellows MLO group (16SrV).
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