ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
PLUM POX VIRUS DIVERSIFICATION OBSERVED IN A SMALL CLUSTER OF ORCHARDS |
| Authors: | L. Quiot-Douine, M. Boeglin, S. Dallot, V. Marie-Jeanne, J.B. Quiot |
| Keywords: | Prunus, Dissemination, Variability, Potyvirus |
| Abstract:
Sixty-six isolates were collected during several years (1992 to 1999), along an east-west transect in a Plum pox potyvirus (PPV) focus located in southern France. Amplification by PCR of both ends of viral coat protein genes, followed by restriction analysis, showed the existence of a PPV natural diversity with a filiation of the variants collected along the transect. These results were confirmed by the micro-sequencing of a selected number of isolates. These data show that an aphid transmissible virus infecting perennial woody plants can induce a sustainable local variability, particularly at the margin of an expanding focus. | |
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