ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
RESISTANCE TO PLUM POX POTYVIRUS IN A TRANSGENIC WOODY PERENNIAL FRUIT TREE, EUROPEAN PLUM (Prunus domestica L.) RESULTS FROM POST-TRANSCRIPTIONAL GENE SILENCING |
| Authors: | R. Scorza, A.M. Callahan, L. Levy, V. Damsteegt, M. Ravelonandro |
| Keywords: | RNA-mediated resistance, Transgene silencing, Post-transcriptional gene silencing |
| Abstract:
Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV and displayed characteristics typical of post-transcriptional gene silencing (PTGS) as described in herbaceous species, including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, and a complex multicopy transgene insertion. | |
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