ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
APPLE CHLOROTIC LEAF SPOT VIRUS IN GERMPLASM COLLECTION OF FRUIT SPECIES |
| Authors: | R. Karesová, F. Paprstein |
| Keywords: | Apples, plums, cherry, ApCLSV, ELISA |
| Abstract:
Samples of apples, plums and cherries were tested for the presence of Apple chlorotic leaf spot virus (ApCLSV) by ELISA on different dates. Samples for testing were collected from different organs of plants, flowers, leaves or bark. Sensitivity of kits for ApCLSV from Bioreba and Sanofi production has been tested. Apple, plum and cherry trees (cultivars) were tested from years 1996 to 1998. A total of 700 trees (apple cultivars) were tested by Bioreba kits for ELISA. Following results have been received: 480 were ApCLSV-positive trees, 57 trees were suspected and only 163 trees were negative. A total of 265 trees of plums were tested. Positive reactions in ELISA were obtained in 94 trees and 7 trees displayed a suspicious reaction. Altogether 278 cherry varieties and clones were tested. Positive test results for the presence of ApCLSV were obtained for 53 trees and 6 have been considered as suspicious. The most suitable organs for detection appeared to be flowers budded at room temperature, and flowers collected from trees in orchard. Tests of stone fruit trees for ApCLSV by ELISA is to be repeated, especially in the case of starting mother materials and materials after therapy procedures. Some of the selected trees with positive results will be maintained and they should serve as a positive check variant in the routine ELISA testing. Both of the commercial kits can be used for ApCLSV testing in cherry trees. Owing to a different sensitivity of kits, both of them are recommended to be simultaneously used in testing of healthy source materials of cherry rootstocks and varieties by ELISA. | |
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