ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
DEVELOPMENT OF PCR PRIMER PAIRS FOR THE CHARACTERIZATION AND DETECTION OF SEVERAL RELATED FILAMENTOUS VIRUSES OF CHERRY |
| Authors: | M.E. Rott, W. Jelkmann |
| Keywords: | CGRMV, CNRMV, CMLV, Cherry mottling, Cherry rusty mottle, Cherry necrotic mottle |
| Abstract:
Cherry can be infected by a number of mottling diseases caused by either filamentous viruses or suspected of being caused by filamentous viruses. The causal agents for two of these diseases have been previously described, Cherry green ring mottle virus (CGRMV) a tentative member of the foveaviruses, and Cherry mottle leaf virus (CMLV) a member of the trichoviruses. In a separate article we also present the complete nucleotide sequence of Cherry necrotic rusty mottle virus (CNRMV). For the mottling diseases, cherry rusty mottle (CRM) and cherry necrotic mottling (CNM), data is presented identifying viruses associated with each disease. Viruses associated with European isolates of cherry mottle leaf (CML) CRM, CNRM and CNM all appear to be closely related to CGRMV and can be tentatively included in the foveavirus genus. An additional, related virus, was discovered during the course of these studies and would appear to be a fairly common latent virus of cherry. It is currently unclear whether this virus is associated with cherry mottling disease (CMot), or is a previously undescribed virus. Several RT-PCR primers are presented for the detection of the different viruses. | |
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