ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
DETECTION AND DIFFERENTIATION OF CHERRY MOTTLE LEAF VIRUS AND PEACH MOSAIC VIRUS |
| Authors: | D. James, C. Upton |
| Keywords: | Peach mosaic virus, Cherry mottle leaf virus, RT-PCR, Simultaneous detection and differentiation |
| Abstract:
Cherry mottle leaf virus (CMLV) and Peach mosaic virus (PMV) are closely related viruses that share common hosts but cause distinct diseases. Nucleotide sequence information, in the region of the replicase gene of CMLV and PMV, was used to design single primer pairs that facilitate simultaneous detection and differentiation of both viruses. A sense primer,100% complementary to the homologous virus and 83% complementary to the heterologous virus at the same site but with 10 perfectly matched bases at the 3' end of the primer, was combined with an antisense primer that was 100% complementary to the homologous virus and 62.5% complementary to the heterologous virus at the alternate downstream site with 5 perfectly matched bases at the 3' end of the antisense primer. This combination of sense and antisense primers was used in RT-PCR for simultaneous detection and differentiation of CMLV and PMV, producing virus specific fragments of 705 bp and 419 bp, respectively. | |
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