ISHS Acta Horticulturae 550: XVIII International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops - Top Fruit Diseases
ISOLATION AND PARTIAL CHARACTERISATION OF A NEW VIRUS INFECTING PRUNUS ARMENIACA (APRICOT) AND BELONGING TO THE NEPOVIRUS GENUS |
| Authors: | P. Gentit, R.P. Delbos, T. Candresse, J. Dunez |
| Abstract:
An extended survey of the sanitary status of Prunus orchards in the south-east of France lead us to collect several budwood samples from a commercial apricot orchard where trees displayed reduced vigour but had no obvious virus-like symptoms on leaves or fruits. Indexing on GF305 peach seedlings in the greenhouse resulted in stunting and other symptoms reminiscent of a Nepovirus infection 1 month after inoculation. The virus was successfully transmitted to herbaceous hosts and then purified using sucrose gradient centrifugation. Under the electron microscope, purified viral particles displayed a typical Nepovirus morphology. The particles contain two single stranded RNAs of 5.9 and 7.9 kb, and a single type of coat protein subunit of 53.7 kDa. By serological analysis, no relationships were found with other Nepoviruses. Partial sequence analysis related this new agent as a putative member of the Nepovirus group. Taken together these results indicate clearly that the virus isolated is a seemingly undescribed Nepovirus. Given the lack of symptoms observed on the original host after back inoculation with purified viral particles, we provisionally named this virus Apricot latent ringspot virus (ALRSV). | |
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