ISHS Acta Horticulturae 547: III International Symposium on Rose Research and Cultivation
SOMATIC EMBRYOGENESIS IN ROSES |
| Authors: | A. Dohm, C. Ludwig, K. Nehring, T. Debener |
| Keywords: | mass propagation, transformation, adventitious shoot formation, callus culture, cell suspension culture, somaclonal variation, AFLP marker |
| Abstract:
Somatic embryogenic callus was initiated from in vitro derived leaf explants following an induction period of four weeks on MS basal medium supplemented with 2,4-D or NAA and several further subcultures on MS medium with zeatine or TDZ. The embryogenic callus was propagated on MS medium containing the phytohormones NAA, zeatine and GA3. For the cultivars ´Heckenzauber´and ´Pariser Charme´ long-term-cultures as well as cell suspensions could be established. Incubation in a 16 h photoperiod was essential for the induction of somatic embryogenesis as well as for callus and embryo development. Although the regeneration protocol originally was developed for the cultivars ´Heckenzauber´ and ´Pariser Charme´, it could be adapted to a broad range of further rose genotypes. Up to now, from 50 tested genotypes 69 % could be regenerated. A critical point in the regeneration process is development of the embryos to plants. Germination occurs only sporadically and the somatic embryos have to be regenerated via adventitious shoot formation. The analysis of about 500 regenerated plants from different cultivars for several morphological traits revealed, that nearly all regenerants were true-to-type. | |
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