ISHS Acta Horticulturae 547: III International Symposium on Rose Research and Cultivation
ANATOMY OF CUT ROSA XYLEM OBSERVED BY SCANNING ELECTRON MICROSCOPE |
| Authors: | H.M.C. Put, A.C.M. Clerkx, D.J. Durkin |
| Abstract:
Two methods for preparation of stem end specimen of Rosa cv. ‘Kardinal’ and Rosa cv. ‘Sonia’ for SEM observations are described SEM is essential in applied laboratory research allowing observations of the rose cut surface and lower parts of the xylem vessels up to 5000 X magnification as shown by photomicrographs. They allow differentiation of water stress of cut roses caused by vessel obstruction with: 1) bacterial particles, 2) particulate matter, 3) macromolecular materials, 4) pectic enzymes, 5) physiological stress without particle obstruction. SEM observations combined with microbiological, biochemical and physiological analysis (water uptake, water potential, water conductivity, acid fuchsin and latex red reactions) contribute in revealing the causes of water stress and vessel obstruction, leading to the development of an effective preventive treatment. | |
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