ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
GENETIC ANALYSIS OF A POLISH APPLE SELECTION U 211 AS A NEW SOURCE OF HIGH RESISTANCE TO APPLE POWDERY MILDEW |
| Authors: | M. Stankiewicz, E. Pitera, S.W. Gawronski |
| Keywords: | marker assisted selection, RAPD, bulked segregant analysis, Malus, Podosphaera leucotricha |
| Abstract:
Breeding apple plants with multiple, durable resistances is an alternative way to control fungal pathogens and reduce any environmental impact due to pesticides use. The breeding of apple cultivars resistant to Podosphaera leucotricha could be much improved by marker-assisted selection. This investigation focuses on the identification of random amplified polymorphic DNA (RAPD) markers linked to the major genes for mildew resistance in a selection U 211 (‘Primula’ open pollinated). The analysis was carried out on 107 plants from progeny ‘Idared’xU211 and parental plants. The cultivar Primula was also analysed. The selection U211 was found to be highly resistant to mildew in field conditions and transmitted a high level of resistance to the majority of its progeny. Moreover the Vf gene from Malus floribunda, conferring resistance to scab, is present in this selection. As the RAPD marker for the Pl1 gene from M. robusta (Markussen et al., 1995) is not present in the DNA of U 211, it is probable that a selection U 211 is a new source of mildew resistance. The RAPD marker technique was combined with a bulked segregant analysis (BSA). About 270 random decamer primers were tested by PCR analysis on the basis of resistant and susceptible DNA pools. Many polymorphisms between two pools were identified. Some of them seem to be useful for marker-assisted selection in apple breeding. | |
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