ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
USE OF DIFFERENT MOLECULAR MARKERS, RAPDS AND SSRS, TO INVESTIGATE CLONAL STABILITY IN MICROPROPAGATED KIWIFRUIT (Actinidia deliciosa A. Chev.) |
| Authors: | M.A. Palombi, C. Damiano |
| Keywords: | kiwifruit, Actinidia spp., DNA fingerprinting, genetic variability |
| Abstract:
Micropropagation has been used to preserve interesting agronomic traits present in Actinidia deliciosa genotypes, maintained in collection at the Istituto Sperimentale per la Frutticoltura. In order to evaluate the clonal stability of micropropagated plants, we compared the efficiency of two molecular markers: RAPD (Random Amplified Polymorphic DNA) and SSR (Simple Sequence Repeat). Polymorphism, both SSR and RAPD, was scored as present (1) or absent (0) fragments and those readings were entered in a computer file as a binary matrix, one for each molecular marker. A cluster analysis, based on the totality of scorable RAPD and SSR bands, was performed to construct two genetic similarity dendrograms, one for each molecular marker. The results indicate that when tissue culture procedure induces genetic variability, its detection depends on the choice of tools for DNA analysis. It is in relation with different polymorphism capability detectable by molecular markers, as our results showed for SSR and RAPD. | |
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