ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
TRANSGENE-BASED RESISTANCE TO PLUM POX VIRUS (SHARKA DISEASE) IS TRANSFERRED THROUGH INTERSPECIFIC HYBRIDIZATION IN PRUNUS |
| Authors: | M. Ravelonandro, P. Briard, R. Renaud, R. Scorza |
| Keywords: | plum pox potyvirus, genetic engineering, protection, breeding. |
| Abstract:
Plum pox virus (PPV) resistant rootstocks would be useful to control the spread of PPV in nurseries and orchards. The PPV-resistant transgenic Prunus domestica plum clone C-5 was used as a parent with Prunus spinosa to develop PPV resistant hybrid progeny. This work showed that C-5 plum is sexually compatible with the Prunus rootstock species P. spinosa. Resistance assays of chip-bud inoculated plants following two cycles of growth and cold-induced dormancy demonstrated that the transgenic hybrid clones were symptomless and DAS-ELISA negative. Further, immuno-blotting failed to detect PPV CP in inoculated transgenic hybrids. These results demonstrated the usefulness of C-5 for developing PPV resistant rootstocks with P. spinosa. | |
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