ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
GENETIC DIVERSITY IN COWPEA (Vigna unguiculata) USING ISOZYME ELECTROPHORESIS |
| Authors: | C.M. Reis, A.M. Frederico |
| Keywords: | cowpea, Vigna unguiculata, cultivar groups, polymorphic loci, esterase |
| Abstract:
Accessions of cultivated cowpea (V. unguiculata ssp. unguiculata cv. gr. unguiculata, cv. gr. sesquipedalis and cv. gr. biflora) from different countries were evaluated for isozyme variability. Zymograms were obtained by polyacrylamide gel electrophoresis using the PhastSystem™ and eight enzyme systems were studied. Cowpea accessions studied were characterised by very low genetic diversity. Accessions were monomorphic for almost all loci tested except for the esterase enzyme system. Comparative analysis of esterase zymograms made it possible to identify some of the accessions studied. However, the cultivated groups biflora and sesquipedalis could not be distinguished from one another or from the cv. gr. unguiculata. | |
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