ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
GENETIC DIVERSITY OF MOROCCAN CULTIVATED OLIVE USING RAPD MARKERS |
| Authors: | B. Khadari, A. Bervillé |
| Keywords: | molecular characterization, Moroccan olive, Olea europaea L., local type, RAPDs |
| Abstract:
An important genetic diversity in cultivated Moroccan olive is expected because of the distribution over a range of environments with contrasting ecological conditions and the history of the country. However, only the Picholine marocaine is described as the variety widely cultivated. In order to assess the genetic diversity in Moroccan cultivated olive, we performed RAPD analysis of olive trees sampled in all traditional areas of olive culture. Using 8 primers, which revealed 45 markers, RAPD analysis of 215 olive trees allowed to identify 68 multilocus profiles. Each of 9 traditional denominations was identified by one specific multilocus profile. In addition, the fact that marocaine has been described as multiclonal variety was not verified since we found only one multilocus profile for 112 olive trees. This multilocus profile displayed in 31 to 93% out of olive sampled in different areas. The same multilocus profile characterize the clones of Picholine Marocaine which are under agronomic evaluation by INRA Morocco (Menara, Haouzia,...). Each one of the remaining 59 multilocus profiles corresponds to olive local type. The dendrogram built according to Ward algorithm and Euclidean distance based on FCA coordinate matrix showed 6 clusters. This study revealed more genotypes than known in cultivated Moroccan olive which correspond to high potential of cultivar selection. | |
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