ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
COMPARISON OF RAPD AND AFLP TECHNIQUES USED FOR THE EVALUATION OF GENETIC DIVERSITY OF CARROT BREEDING MATERIALS |
| Authors: | D. Grzebelus, R. Baranski, B. Jagosz, B. Michalik, P.W. Simon |
| Keywords: | Daucus carota L., F1 hybrids, molecular markers, PCR, correlation |
| Abstract:
The objective of the present study was to compare two of the most widely used PCR-based techniques, i.e. RAPD and AFLP. Standard protocols were applied to identify markers for 12 genotypes, including four sets of breeding lines (male-sterile and the corresponding maintainer) and three F1 hybrids. Fifteen of 33 screened RAPD primers produced clear and consistent banding patterns, yielding in the total of 47 markers. Eight AFLP primer combinations produced 88 markers. Both analysed techniques allowed the evaluation of genetic diversity for the screened carrot breeding materials. The dendrograms represented a similar layout, closely approximating the known pedigree data. However, the values of genetic similarity for the tested techniques were different. The analysis of correlation between the genetic similarity matrices confirmed a high level of consistency for the results obtained with RAPD and AFLP data. Pearson linear correlation coefficient reached 0.71 while Spearman rank correlation coefficient was 0.55, both being highly significant. The combination of the two sets of data produced a dendrogram more closely representing the actual relationships between the used carrot materials. | |
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