ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
APPLICATION OF MICROSATELLITE-BASED DNA PROFILING IN ACTINIDIA SPECIES |
| Authors: | L.G. Fraser, C.F. Harvey, G.P. Gill |
| Keywords: | DNA profiling, DNA fingerprinting, microsatellite, Actinidia |
| Abstract:
Actinidia, a genus of dioecious vines, contains several species that bear fruit of economic value, among them the familiar kiwifruit. We have applied a set of nine microsatellite markers to leaf material of three Actinidia species, at three different ploidy levels. At each level parental and sibling plants were used to test the ability of the marker set to distinguish particular genotypes from closely related material. Eight of the nine markers, capable of distinguishing cultivars related to Actinidia deliciosa cv. Hayward, hexaploid, were polymorphic in an A. deliciosa family, and eight were also polymorphic in a family of the closely related diploid species Actinidia chinensis. In the more distantly related Actinidia arguta, only four of the nine markers were informative. In the three species the marker kit did distinguish individual genotypes in each family. | |
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