ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
GENOTYPE IDENTIFICATION OF ORNAMENTAL SPECIES BY RAPD ANALYSIS |
| Authors: | L. De Benedetti, A. Mercuri, S. Bruna, G. Burchi, T. Schiva |
| Keywords: | genetic fingerprinting, Limonium spp, Prunus spp., Carnation (Dianthus caryophillus), rose (Rosa hybrida), Gerbera (Gerbera jamesonii hybrida |
| Abstract:
The use of highly discriminatory methods for the identification and characterisation of genotypes is essential for breeding programmes and for plant patent protection. RAPD (Random Amplified Polymorphic DNA) markers have been found to be very useful in studying the genetic variability of different species, including ornamentals. We have utilised the RAPD methodology for the genetic fingerprinting of Limonium, ornamental Prunus and carnation. The genotypes analyzed belong to our collection and are currently utilised in our breeding programmes. Different cultivars of rose and Gerbera, supplied from private breeders, were also analysed for variety discrimination. DNA extraction protocols and PCR conditions were established. The random primers that gave the better amplification patterns were also determined. The results obtained allowed to discriminate every genotype of the different species examined and to identify the cultivars analysed. | |
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