ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
CHARACTERISATION OF THE MOST IMPORTANT SPANISH GRAPE VARIETIES THROUGH ISOZYME AND MICROSATELLITE ANALYSIS |
| Authors: | J. Borrego, M.T. Rodriguez, J. Martin, J. Chavez, F. Cabello, J. Ibañez |
| Keywords: | Vitis vinifera, SSRs, cultivar-identification, fluorescence, synonymies, homonymies, ampelography |
| Abstract:
The aim of this work was to clarify the viticultural view in Spain, where there are many cultured varieties and many names for each variety. A morphological study was previously performed to clear the way. Then two molecular techniques were used to solve the numerous doubts still present: isozyme and microsatellite analyses. Four different systems were studied during isozyme analysis: catechol oxidase, acid phosphatase, glutamate-oxalacetate transaminase and superoxide dismutase. The microsatellites, analysed using fluorescent markers, were VVS2, VVS5, VVS29, VVMD5, VVMD7, ssrVrZAG 47, ssrVrZAG 62 and ssrVrZAG 79. Initially 406 accessions were studied, which originally were grouped into 47 different varieties. These accessions included the most important varieties grown in Spain. Isozyme and microsatellite analysis has raised the number of different varieties to 116. This study showed that many accessions initially included in the same variety were in fact different, while others, presumed to be different varieties, were in reality the same. | |
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