ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
MOLECULAR MARKERS IN DETECTION OF DISTANT HYBRIDS IN LILIUM |
| Authors: | K. Wiejacha, A. Marasek, I. Sabala, T. Orlikowska |
| Keywords: | DNA polymorphism, distant hybridisation, Lilium henryi, L. pumilum, DNA polymorphism, distant hybridisation, Lilium henryi, L. pumilum, |
| Abstract:
Plants were obtained by ovule rescue from pollinations of lily cultivars belonging to the group "oriental hybrids" with pollen of Lilium henryi, L. pumilum and L. x formolongi. In order to develop molecular DNA markers useful for separation of the hybrids all the parental genotypes, resulting seedlings and regenerants were fingerprinted using RAPDs. The products of amplification were detected for all the forty primers used. Eight primers generated reproducible polymorphic bands within the pair 'Marco Polo' and L. henryi, six primers within 'Alma Ata' and L. pumilum, and 'Muscadet' and L.x formolongi. It was found that some bands could serve as markers to detect hybrids at the in vitro stage | |
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