ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
EVALUATION OF GENETIC RELATIONSHIPS IN CITRUS GENUS BY MEANS OF SEQUENCE TAGGED MICROSATELLITES |
| Authors: | F. Luro, D. Rist, P. Ollitrault |
| Keywords: | SSR, evolution, genetic distance, allelic diversity, taxonomy |
| Abstract:
Simple Sequence Repeats (SSRs) have proven to be useful genetic markers in a wide variety of plants, but have not yet to be widely applied to Citrus identification. In this study, genetic relationships among 73 individuals of 8 more economically important species of the Citrus genus (C. reticulata Blanco, C. sinensis (L.) Osb., C. aurantium L., C. paradisi Macf., C. maxima (Burm.) Merr., C. limon (L.) Burm., C. aurantifolia (Christm.) Swing. and C. medica L.) were estimated by the amplification of 10 loci of SSR. The potential of STMS for cultivar identification within species has been evaluated and a neighbour joining analysis on the basis of a dissimilarity matrix among the different genotypes has been performed. Polymorphism of 8 STMS was sufficient so as to obtain genetic aggregations where three main groups were clearly identified: the orange-mandarin group, the pummelo-grapefruit group and the lime-citron-lemon group. These Citrus species relationships were in large agreement with those established by previous analyses based on molecular markers and morphological traits, suggesting that SSR data will be useful for exploring infra-generic Citrus genetic taxonomy. The studied microsatellites have not enabled varieties within some of the species, known to have evolved by somatic mutations to be distinguished, such as sweet oranges, grapefruits and sour oranges. However polymorphism has been detected among lemon varieties. | |
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