ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
RAPD AND AFLP GENETIC MARKERS FOR THE CHARACTERISATION OF OSTEOSPERMUM GERMPLASM |
| Authors: | T. Berio, G. Morreaale, A. Giovannini, L. Allavena, L. Arru, P. Faccioli, V. Terzi, N. Pecchioni |
| Keywords: | African Daisy, molecular markers, genetic similarity, biodiversity |
| Abstract:
The level of genetic diversity was assayed within the accessions of three breeding groups in an Osteospermum collection. The DNA-profiling via RAPD revealed high levels of polymorphism. Genetic variation was highest in the English material, intermediate in the Italian and lowest in the Danish breeding group. The most frequent similarity classes were 0.5-0.6 for the English, 0.6-0.7 for the Italian and 0.7-0.8 for the Danish group. Furthermore, an AFLP analysis has been carried out on a sample of eight accessions representative of the three groups. Preliminary results indicate that AFLP analysis is suitable to give polymorphism in Osteospermum. RAPD and AFLP analysis can therefore be a useful tool for evaluating genetic diversity for breeding purposes, and for protecting intellectual property rights of new varieties. | |
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