ISHS Acta Horticulturae 546: International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
USE OF AFLP MARKER TECHNIQUE FOR CLASSIFICATION OF ALSTROEMERIA SPP. AND CONSTRUCTION OF A LINKAGE MAP IN ALSTROEMERIA AUREA |
| Authors: | T.H. Han, H.J. van Eck, M.J. de Jeu, E. Jacobsen |
| Keywords: | AFLP fingerprinting, cluster analysis, genetic diversity, linkage map, ornamental crop, principal co-ordinate analysis |
| Abstract:
Alstroemeria, also known as the Peruvian or Inca lily is a genus in the family of Alstroemeriaceae. It is an economically important cut flower in the Netherlands. The nuclear DNA content (2C-value) ranges from 37,000 to 79,000 Mbp for Chilean species and from 50,000 to 56,000 Mbp for Brazilian species. This huge genome size required adaptation of the AFLP protocol by increasing the number of selective nucleotides for preamplification and final amplification. Reliable fingerprints were obtained with a pre-amplification with EcoRI+2/MseI+2 primers and a final amplification with EcoRI+4/MseI+4 primers. Our preliminary results with another enzyme combination (Sse8387I and MseI; Sse8387I has a recognition sequence of 8 bp) are discussed. We produced AFLP fingerprints of 22 Alstroemeria species, one interspecific hybrid (A. aurea ´ A. inodora) and the distantly related species Bomarea salsilla and Leontochir ovallei and we analysed their genetic relationships. Both cluster analysis and principal co-ordinate analysis could separate the Chilean species, the Brazilian species, and the outgroup species. Within the group of Chilean species, two clusters of related species could be identified. The first subgroup contains A. pelegrina and A. umbellata. The second subgroup contains A. ligtu incarnata, A. haemantha, A. ligtu ligtu, and A. ligtu simsii. Alstroemeria aurea is found between the cluster of Brazilian and Chilean species. A genetic linkage map has been constructed using the F1 offspring (N=134) of two non-inbred parental genotypes of A. aurea | |
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