ISHS Acta Horticulturae 545: V International Protea Research Symposium
AN ANATOMICAL STUDY OF ADVENTITIOUS ROOT DEVELOPMENT IN WOUNDED CUTTINGS OF LEUCADENDRON DISCOLOR AND LEUCADENDRON ’SAFARI SUNSET’ (PROTEACEAE) |
| Authors: | J.F. Pérez-Francés, M.N. Melián-Capote, J.A. Rodriguez-Perez |
| Keywords: | callus, histology, root initiation |
| Abstract:
Terminal cuttings (about 15 cm length) were used for experiments. Two shallow and opposite incisions were made with a sharp knife in the basal bark. The bases of the cuttings were dipped in 4000 ppm indolyl-butyric acid in 50 per cent ethanol for 5 seconds followed by a dip in talc containing benomyl and captan, and then, planted in a suitable substrate. Rooting was obtained in the majority of cuttings after three weeks. The main anatomical changes associated with adventitious root development in wounded cuttings appear to be similar in Leucadendron discolor and Leucadendron ´Safari Sunset´. Nodular callusing from cortical and cambial cells was formed in the wound and basal cut surface, and it seem to be important to the initiation of root primordia. Callus initiation was observed after one week from treatment. Adventitious root formation was mainly located in the wounded area and in the basal cut surface. However, some roots were developed from undamaged tissues. After 2 weeks from cutting treatment, the initiation of root primordia were detected in wound calli. Groups of differenciated vascular cells with some orientation along the main axis were observed in an early developmental stage of primordia. | |
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