ISHS Acta Horticulturae 545: V International Protea Research Symposium
IN VITRO ESTABLISHMENT AND PROLIFERATION OF AXILLARY BUD CULTURES OF LEUCADENDRON DISCOLOR (PROTEACEAE) |
| Authors: | J.F. Pérez-Francés, B.J. Ravelo, J.A. Rodríguez-Pérez |
| Keywords: | micropropagation, tissue culture, Proteaceae |
| Abstract:
A method for the in vitro establishment and multiplication of Leucadendron discolor through axillary shoot development is described. Nodal explants and shoot tips were used as starting material. The best explants were partially lignified nodal segments collected from growing branches of field-grown plants. Polyvinyl-pyrrolidone at 10-4 M was applied to avoid oxidation. A seasonal effect was observed in explant viability with the best responses observed during spring. For establishment phase, nodal explants were cultured on Murashige and Skoog medium at half strength (1/2 MS) with 3% sucrose, 0.7% agar and 0.5 mg/l benzyladenine. After thirty days, explants developed axillary buds and were subcultured to fresh medium of the same composition. After thirty days, elongated buds were excised from primary explants and transferred to a proliferation medium. The medium used during establishment phase also produced suitable shoot proliferation. The use of a double-phase system or addition of auxin to the medium during multiplication phase did not significantly improve explant response. The proliferation ability was maintained by transferring shoots (after 30-40 days) to fresh medium. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
