ISHS Acta Horticulturae 542: VII International Symposium on the Processing Tomato
THREE-YEAR RISK ASSESSMENT OF THE PRACTICAL APPLICATION OF CROSS-PROTECTION IN PROCESSING TOMATO FIELDS USING AN ATTENUATED CUCUMBER MOSAIC VIRUS (CMV) STRAIN CONTAINING AN AMELIORATIVE SATELLITE RNA |
| Authors: | H. Sayama, M. Kominato, M. Ubukata, T. Sato |
| Keywords: | Vaccination, preinoculation, biological control, Cucumovirus, Lycopersicon esculentum, mild strain, Japan |
| Abstract:
Since 1995 we have used preinoculation (the term vaccination is used below) of tomato seedlings with a mild satellite RNA (satRNA)-containing CMV strain KO3 on a practical scale to protect processing tomato fields against virulent CMV. Although no incidents or damage have occurred from this practice thus far, in the past 3 years we have carried out risk assessment studies by systematically comparing vaccinated and non-vaccinated tomato fields. During the 3-year period non-vaccinated fields consistently had higher ratios of virus-infected plants with more severe symptomatology than did the vaccinated fields. In 1999 CMV samples from non-vaccinated fields contained two sizes of satRNAs: 30% of the samples contained the 390nt and 18% the 340nt length class of CMV satRNA, while 5% had a mixture of the two classes. The remaining samples (47%) contained no satRNA. All CMV samples from vaccinated fields contained only one 390nt satRNA, which we believe to have been derived from the 391nt satRNA of CMV-KO3. In 1997 we detected no satRNA in samples from 285 overwintered weed plants representing 7 different species. Collectively the data show no clear adverse risk in association with satellite-mediated cross-protection technology. | |
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