ISHS Acta Horticulturae 541: IV International Symposium on New Floricultural Crops
IN VITRO INDUCTION OF ADVENTITIOUS ROOTS AND PROLIFERATION OF CISTUS CRETICUS CRETICUS L. PLANTS |
| Authors: | Z. Pela, M. Pentcheva, D. Gerasopoulos, E. Maloupa |
| Keywords: | Seedlings, explants, lateral buds, shoot tips, plant propagation, rooting, auxins |
| Abstract:
The effect of two auxins (NAA and 2,4-D) and BAP (at 0.89µM) on in vitro adventitious root induction and callus induction from shoot tips and lateral buds of Cistus creticus creticus explants 0, 1.136, 2.272, and 4.545 mg.L-1 6- benzylaminopurine (BAP) combined with 0 or 0.175 mg.L-1 of IAA or 0.188 mg.L-1 NAA was studied. Cultures were initiated on MS medium supplemented with 0.89µM BAP, from shoot tips and nodal segments of three-week seedlings obtained in vitro. Microshoots (»1 cm in length) after the second subculture were successfully rooted on MS + 2,4-D or NAA at different concentrations. Part of the shoot-tip and nodal explants were left on the same multiplication medium (MS + 0.89 µM BAP + activated charcoal 0.25 g.L-1) for 7 weeks, and showed the highest rooting percentage, root length, and root number. Cistus creticus creticus microshoots also formed adventitious roots on a medium without of plant growth regulators. Shoot proliferation and lateral bud or shoot tip explants were cultured for 5 weeks in media containing 2.272 mg L-1 BAP produced 3 shoots and 11 leaves, or 2.5 shoots and 8 leaves per explant, respectively. Proliferation of lateral shoots taken from the lateral buds explant was higher compared to proliferation of lateral shoots arising from shoot tip explants. Callus obtained from shoot tips and lateral buds cultured in media containing 2.272 mg L-1 BAP and 0.175 mg L-1 IAA had mean diameter of 10.86 and 20.4 mm, respectively. Mean fresh weight and dry weight of callus obtained from lateral buds or shoot tips used as explants, were 238.47 mg and 20.471 mg or 226.03 mg and 25.43 mg in media containing 1.136 mg BAP combined with 0.175 mg IAA in media, respectively. | |
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