ISHS Acta Horticulturae 541: IV International Symposium on New Floricultural Crops
IN VITRO REGENERATION OF EBENUS CRETICA, L. |
| Authors: | J.C. Vlahos, M. Dragassaki |
| Keywords: | Endemic, ornamental, rooting, vitrification |
| Abstract:
Ebenus cretica, L , Fabaceae is an attractive endemic plant of Crete being studied at the TEI of Heraklion with promising results as a potential new flower crop. Research has shown that E.cretica can be propagated by seed or stem cuttings. However, due to great variability observed among the native population, a series of experiments have been initiated for in vitro shoot proliferation and rooting of selected ebenus plants with desirable characteristics. Preliminary experiments in vitro with explants taken from field or greenhouse growing plants resulted in high infection rates. Therefore, E.cretica seeds were germinated in vitro for providing sterile plant material. Highest percentage of germination was observed on a half strength MS medium (pH =5.8) at 220 C and 16h light in three weeks. 1 cm long shoot tip explants were taken from 10 week old plantlets and placed on MS , Lepoivre and Nitch media. Highest shoot proliferation was recorded when 0.1 mg/L BAP and 0.1 mg/L NAA were added to MS. When shoots were transferred to MS medium supplemented with 1 or 1.5 mg/L IBA rooting was enhanced. In all treatments callus growth was observed and vitrification symptoms were evident in several of the treatments. Additionally, anther culture in vitro has been carried out for production of haploid plants with limited success so far. Further investigations are required in order to establish a successful in vitro regeneration of Ebenus cretica. | |
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