ISHS Acta Horticulturae 541: IV International Symposium on New Floricultural Crops
PRELIMINARY STUDY ON LARGE SCALE IN VITRO PROPAGATION OF MYRTUS COMMUNIS L. |
| Authors: | K. Grigoriadou, N. Leventakis |
| Keywords: | Myrtus communis, in vitro, micropropagation, acclimatization |
| Abstract:
In vitro production of multiple shoots, in vitro and ex vitro rooting and acclimatization were examined. Approximately 3 shootlets/explant were achieved after 4 weeks of culture in MS basic salts supplemented with 3µM BAP, 0.3µM GA3, 0.05µM NAA, 20% sucrose (w/v) and 0.5% (w/v) agar. The addition of 5.4µM of NAA and 5.0 µM IBA in MS basic medium resulted in 70% rooting. Rooted shoots were planted in 1:1 peat-perlite under 90%RH. Acclimatization proved to be problematic under a fog system. However, ex vitro rooting was also attempted, 55% of the microcuttings gave rise to new plantlets, after immersion in 2000ppm IBA. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
