ISHS Acta Horticulturae 540: I International Conference on Banana and Plantain for Africa
APPLICABILITY OF AN AGROBACTERIUM-BASED SYSTEM FOR THE TRANSFORMATION OF MUSA SPECIES WITH DIVERSE GENOMIC CONSTITUTION AND PLOIDY LEVEL |
| Authors: | N.A. Bosque-Pérez, G.D. May, C.J. Arntzen |
| Keywords: | Agrobacterium tumefaciens, cooking bananas, dessert bananas, plantains, plant transformation |
| Abstract:
An Agrobacterium-mediated transformation protocol was tested using eight clones of Musa with different ploidy and genomic background. These were selected to represent several major groups of Musa including dessert and highland bananas (AAA), cooking bananas (ABB), improved plantain hybrids (AAB X AA) and wild diploids (AA and BB). Following co-cultivation, GUS histochemical activity was detected in tissue of the highland banana cultivar Mbwazirume and the wild diploid Musa balbisiana. For the remaining clones, GUS enzymatic assays indicated that the gus reporter gene was expressed in putative transformants. Expression of the npt-II gene was also detected. Our results confirm that A. tumefaciens is able to infect Musa spp. and that it can be used with Agrobacterium-based vectors for foreign gene transfer into bananas and plantains. | |
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