ISHS Acta Horticulturae 539: III International Symposium on Brassicas and XII Crucifer Genetics Workshop
CHANGES IN MITOCHONDRIAL AND CHLOROPLAST GENOME STRUCTURE ACCOMPANIED WITH CYTOPLASMIC MALE STERILITY INDUCED BY PROTOPLAST FUSION AND CHIMERA SYNTHESIS IN BRASSICACEAE |
| Authors: | Y. Hirata, T. Motegi, Y. Hogi |
| Keywords: | cytoplasmic male sterility, mitochondria, chloroplast, chimera, protoplast fusion, Brassicaceae |
| Abstract:
We obtained cytoplasmic male sterile (CMS) lines by artificial chimera between red cabbage and Komatsuna (B. campestris), and by intergeneric protoplast fusion between radish (Raphanus sativus) and cabbage (Brassica oleracea). Two types of different CMSs have stably maintained the radish CMS-specific orf138 in the mitochondria, although parental materials in both CMSs are normal plants. As it is very difficult to explain the mechanism of recombination within each cytoplasmic genome, the change in quantitative regulation was thought to be due to the copy number of heteroplasmic genomes or genes that were originally present. RFLP and PCR analyses support the existence of stoichiometric shifts in mitochondrial and chloroplast genomes or genes. In order to determine the stoichiometric shifts of cytoplasmic genomes accompanying CMS induction, RFLP patterns among mother plants and the resulting CMSs were compared using known chloroplast and mitochondrial gene probes. RFLPs in the two CMSs were very similar to those in Ogura CMS radish. PCR cycle experiments also supports our theory. | |
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